Impact of indium-111 oxine labelling on viability of human mesenchymal stem cells in vitro, and 3D cell-tracking using SPECT/CT in vivo.
Identifieur interne : 001370 ( Main/Exploration ); précédent : 001369; suivant : 001371Impact of indium-111 oxine labelling on viability of human mesenchymal stem cells in vitro, and 3D cell-tracking using SPECT/CT in vivo.
Auteurs : RBID : pubmed:21080231English descriptors
- KwdEn :
- Animals, Cell Differentiation, Cell Line, Cell Movement, Cell Survival, Cell Tracking (methods), Female, Humans, Immunohistochemistry, Mesenchymal Stromal Cells (cytology), Mesenchymal Stromal Cells (metabolism), Organometallic Compounds (metabolism), Oxyquinoline (analogs & derivatives), Oxyquinoline (metabolism), Rats, Nude, Staining and Labeling, Tomography, Emission-Computed, Single-Photon, Tomography, X-Ray Computed.
- MESH :
- chemical , analogs & derivatives : Oxyquinoline.
- chemical , metabolism : Organometallic Compounds, Oxyquinoline.
- cytology : Mesenchymal Stromal Cells.
- metabolism : Mesenchymal Stromal Cells.
- methods : Cell Tracking.
- Animals, Cell Differentiation, Cell Line, Cell Movement, Cell Survival, Female, Humans, Immunohistochemistry, Rats, Nude, Staining and Labeling, Tomography, Emission-Computed, Single-Photon, Tomography, X-Ray Computed.
Abstract
This study investigates the effects of (111)In-oxine incorporation on human mesenchymal stem cells' (hMSC) biology and viability, and the applicability of (111)In-oxine for single-photon emission computed tomography/X-ray computed tomography (SPECT/CT) monitoring of hMSC in vivo.
DOI: 10.1007/s11307-010-0439-1
PubMed: 21080231
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Impact of indium-111 oxine labelling on viability of human mesenchymal stem cells in vitro, and 3D cell-tracking using SPECT/CT in vivo.</title><author><name sortKey="Gildehaus, Franz Josef" uniqKey="Gildehaus F">Franz Josef Gildehaus</name><affiliation wicri:level="3"><nlm:affiliation>Department of Nuclear Medicine, University of Munich (LMU), Munich, Germany. franz.gildehaus@med.uni-muenchen.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Nuclear Medicine, University of Munich (LMU), Munich</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName></affiliation></author><author><name sortKey="Haasters, Florian" uniqKey="Haasters F">Florian Haasters</name></author><author><name sortKey="Drosse, Inga" uniqKey="Drosse I">Inga Drosse</name></author><author><name sortKey="Wagner, Erika" uniqKey="Wagner E">Erika Wagner</name></author><author><name sortKey="Zach, Christian" uniqKey="Zach C">Christian Zach</name></author><author><name sortKey="Mutschler, Wolf" uniqKey="Mutschler W">Wolf Mutschler</name></author><author><name sortKey="Cumming, Paul" uniqKey="Cumming P">Paul Cumming</name></author><author><name sortKey="Bartenstein, Peter" uniqKey="Bartenstein P">Peter Bartenstein</name></author><author><name sortKey="Schieker, Matthias" uniqKey="Schieker M">Matthias Schieker</name></author></titleStmt><publicationStmt><date when="2011">2011</date><idno type="doi">10.1007/s11307-010-0439-1</idno><idno type="RBID">pubmed:21080231</idno><idno type="pmid">21080231</idno><idno type="wicri:Area/Main/Corpus">001697</idno><idno type="wicri:Area/Main/Curation">001697</idno><idno type="wicri:Area/Main/Exploration">001370</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Differentiation</term><term>Cell Line</term><term>Cell Movement</term><term>Cell Survival</term><term>Cell Tracking (methods)</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>Mesenchymal Stromal Cells (cytology)</term><term>Mesenchymal Stromal Cells (metabolism)</term><term>Organometallic Compounds (metabolism)</term><term>Oxyquinoline (analogs & derivatives)</term><term>Oxyquinoline (metabolism)</term><term>Rats, Nude</term><term>Staining and Labeling</term><term>Tomography, Emission-Computed, Single-Photon</term><term>Tomography, X-Ray Computed</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Oxyquinoline</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Organometallic Compounds</term><term>Oxyquinoline</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Mesenchymal Stromal Cells</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mesenchymal Stromal Cells</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cell Tracking</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Differentiation</term><term>Cell Line</term><term>Cell Movement</term><term>Cell Survival</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>Rats, Nude</term><term>Staining and Labeling</term><term>Tomography, Emission-Computed, Single-Photon</term><term>Tomography, X-Ray Computed</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study investigates the effects of (111)In-oxine incorporation on human mesenchymal stem cells' (hMSC) biology and viability, and the applicability of (111)In-oxine for single-photon emission computed tomography/X-ray computed tomography (SPECT/CT) monitoring of hMSC in vivo.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">21080231</PMID><DateCreated><Year>2011</Year><Month>11</Month><Day>09</Day></DateCreated><DateCompleted><Year>2012</Year><Month>03</Month><Day>15</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1860-2002</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>6</Issue><PubDate><Year>2011</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging</Title><ISOAbbreviation>Mol Imaging Biol</ISOAbbreviation></Journal><ArticleTitle>Impact of indium-111 oxine labelling on viability of human mesenchymal stem cells in vitro, and 3D cell-tracking using SPECT/CT in vivo.</ArticleTitle><Pagination><MedlinePgn>1204-14</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11307-010-0439-1</ELocationID><Abstract><AbstractText Label="PURPOSE" NlmCategory="OBJECTIVE">This study investigates the effects of (111)In-oxine incorporation on human mesenchymal stem cells' (hMSC) biology and viability, and the applicability of (111)In-oxine for single-photon emission computed tomography/X-ray computed tomography (SPECT/CT) monitoring of hMSC in vivo.</AbstractText><AbstractText Label="PROCEDURES" NlmCategory="METHODS">HMSC were labelled with 10 Bq/cell. Cellular retention of radioactivity, cell survival, and migration were evaluated over 48 h. Metabolic activity was assessed over 14 days and the hMSC's stem cell character was evaluated. Serial SPECT/CT was performed after intra-osseous injection to athymic rats over 48 h.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Labelling efficiency was 25%, with 61% of incorporated (111)In remaining in the hMSC at 48 h. The radiolabelling was without effect on cell viability, stem cell character, and plasticity, whereas metabolic activity and migration were significantly reduced. Grafted cells could be imaged in situ with SPECT/CT.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">(111)In-oxine labelling moderately impaired hMSC's functional integrity while preserving their stem cell character. Combined SPECT/CT imaging of (111)In-oxine-labelled hMSC opens the possibility for non-invasive sequential monitoring of therapeutic stem cells.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gildehaus</LastName><ForeName>Franz Josef</ForeName><Initials>FJ</Initials><Affiliation>Department of Nuclear Medicine, University of Munich (LMU), Munich, Germany. franz.gildehaus@med.uni-muenchen.de</Affiliation></Author><Author ValidYN="Y"><LastName>Haasters</LastName><ForeName>Florian</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Drosse</LastName><ForeName>Inga</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Wagner</LastName><ForeName>Erika</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Zach</LastName><ForeName>Christian</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Mutschler</LastName><ForeName>Wolf</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Cumming</LastName><ForeName>Paul</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Bartenstein</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Schieker</LastName><ForeName>Matthias</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Imaging Biol</MedlineTA><NlmUniqueID>101125610</NlmUniqueID><ISSNLinking>1536-1632</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Organometallic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>14514-42-2</RegistryNumber><NameOfSubstance>indium oxine</NameOfSubstance></Chemical><Chemical><RegistryNumber>5UTX5635HP</RegistryNumber><NameOfSubstance>Oxyquinoline</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Differentiation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Movement</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Survival</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Tracking</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Immunohistochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mesenchymal Stromal Cells</DescriptorName><QualifierName MajorTopicYN="Y">cytology</QualifierName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Organometallic Compounds</DescriptorName><QualifierName MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Oxyquinoline</DescriptorName><QualifierName MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rats, Nude</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Staining and Labeling</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Tomography, Emission-Computed, Single-Photon</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Tomography, X-Ray Computed</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>11</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>11</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>3</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1007/s11307-010-0439-1</ArticleId><ArticleId IdType="pubmed">21080231</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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